Diffuse large B cell lymphomas relapsing in the CNS lack oncogenic MYD88 and CD79B mutations

Diffuse large B cell lymphoma (DLBCL) is a clinically and molecularly heterogeneous disease. The majority of patients respond to immunochemotherapy, generally consisting of rituximab in combination with CHOP (cyclophosphamide, doxorubicin, vincristine, prednisone) and 60–70% can be cured. Approximately 30% will develop a relapse, of which relapses in the central nervous system (CNS), although relatively rare (~1%), carry a particularly poor prognosis. The factors that determine homing to extranodal sites such as the CNS are largely unknown, but a much higher incidence of CNS relapse is observed in very aggressive lymphoma types such as Burkitt lymphoma, lymphoblastic lymphoma, and primary testicular lymphoma (PTL). We and others have previously demonstrated that primary CNS lymphomas (PCNSLs) and PTLs, both arising at immune-privileged sites, are characterized by a high frequency of oncogenic mutations in both CD79B, causing chronic active B cell receptor (BCR) signaling, and in MYD88, an adapter protein that mediates toll-like receptor (TLR) and interleukin-1 receptor signaling. Both mutations ultimately lead to activation of the NF-kB pathway. Although they are found almost exclusively in activated B cell type (ABC-type) DLBCL, there is a striking difference in the prevalance of MYD88 mutations in PCNSL (75%) and PTLs (71%) versus nodal lymphomas (17%) and gastrointestinal (11%) lymphomas. To explore whether MYD88 and CD79B mutations are preferentially associated with DLBCL originating in the CNS or testis, or whether they are also present in DLBCL relapsing in the CNS, we tested a panel of 14 patients with CNS relapse of a DLBCL. These patients, with either leptomeningeal and/or brain parenchymatous relapse, were treated in the phase II HOVON 80 NHL trial with reinduction chemotherapy (consisting of three cycles of R-DHAPMTX (dexamethasone 40mg on days 1–4, cisplatin 100mg/m on day 1, cytarabine 2 × 2 g/m on day 2, rituximab 375mg/m on day 5, methotrexate 3 g/m on day 15) and intrathecal rituximab (registered at www.trialregister.nl as NTR1757, EudraCT number 2006-002141-37). Patients with either a partial or a complete response received consolidation with busulfan/cyclophosphamide and autologous stem cell transplantation; all others went off protocol. A total of 36 eligible patients, aged 23–65 years (median 57 years) were treated between 2007 and 2011, 24 of whom had parenchymal localizations on MRI and 18 of whom had a leptomeningeal relapse. The overall response rate for these patients was 53% (28% of patients reached a complete response), with a median response duration of 6 months. Mutation analysis was performed in the 14 patients for whom either brain biopsy material or tumor-positive cerebrospinal fluid (on the basis of pathology and/or immunophenotyping results) was available. For 13 of these patients the biopsy material obtained at primary diagnosis could also be retrieved. We used a panel of allele-specific PCRs covering all major mutation (hot) spots to detect somatic mutations in MYD88 and CD79B. As recently reported, this strategy permits efficient and sensitive detection of mutations. The detected mutations were verified by Sanger sequencing. None of the 27 tested samples (13 primary material, 14 relapse material) showed CD79B mutations and a MYD88 mutation was found in 3/14 CNS relapse patients only (21%; Table 1).